ENCBS242UJR / primary cell

Summary

Status
released
Term name
activated CD4-positive, alpha-beta T cell
Term ID
CL:0000896
Summary
Homo sapiens male adult (35 years) activated CD4-positive, alpha-beta T primary cell treated with 10 ng/mL Interleukin-2 for 14 days, anti-CD3 and anti-CD28 coated beads for 24 hours, anti-CD3 and anti-CD28 coated beads for 14 days
Starting amount
500000cells
Separated from biosample
ENCBS862RAU

Attribution

ENCODE4 project
Lab
Yijun Ruan, JAX
Award PI
Yijun Ruan, JAX
Submitted by
Bonita Lam
Source
AllCells
Project
ENCODE
External resources
Aliases
barbara-wold:CD4-Naive-Th0-Day13-Activation_LP-18_2

Treatment details

Treatment
10 ng/mL Interleukin-2 (UniProtKB:P60568) for 14 days
Type
protein
Purpose
stimulation
Treatment
anti-CD3 and anti-CD28 coated beads for 24 hours
Type
antibody
Purpose
activation
Treatment
anti-CD3 and anti-CD28 coated beads for 14 days
Type
antibody
Purpose
activation

Donor information

Status
released
Accession
ENCDO374BBL
Aliases
yijun-ruan:donor_LP-18
Donor external identifiers
  • AllCells:10477
Species
Homo sapiens
Life stage
adult
Age
35 years
Sex
male
External resources
Submitter comment
AllCells:10477

Documents

cell isolation protocol
Description excerpt: Protocol describing the purification, activation, and differentiation of…
Description
Protocol describing the purification, activation, and differentiation of various immune cells. This document is an updated version of /documents/f1b60b4e-b5d2-4b8d-af0b-06b767fa07c3/.
Submitted by
Jennifer Jou
Lab
Charles Lee