Experiment summary for ENCSR293VAR
doi:10.17989/ENCSR293VAR
Summary
- Status
- released
- Assay
- CRISPR genome editing followed by RNA-seq (CRISPR RNA-seq)
- Target
- SPAG9
- Biosample summary
- Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens SPAG9
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- RNA-seq on HepG2 cells treated with a CRISPR gRNA against SPAG9. (SPAG9-BGHcLV31)
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 9.9 [1-1]9.8 [2-1]
- Size range
- >200
- Extraction method
- Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR156CIL
- Platform
- Illumina NovaSeq 6000
- Controls
Attribution
ENCODE4 project
- Lab
- Brenton Graveley, UConn
- Award
- U41HG009889 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:SPAG9-BGHcLV31
- Date released
- October 27, 2022
- Gene Silencing Series
- ENCSR210JCP
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens HepG2 cell line genetically modified (deletion) using CRISPR targeting H. sapiens SPAG9 | ENCBS657JNU | ENCGM117FSW | ENCLB100TVZ |
| 2 | 1 | Homo sapiens HepG2 cell line genetically modified (deletion) using CRISPR targeting H. sapiens SPAG9 | ENCBS558TFT | ENCGM117FSW | ENCLB297GIP |