ENCBS558TFT / cell line
Summary
- Status
- released
- Term name
- HepG2
- Term ID
- EFO:0001187
- Summary
- Homo sapiens HepG2 cell line genetically modified (deletion) using CRISPR targeting H. sapiens SPAG9
- Description
- RNA-seq on HepG2 cells treated with a CRISPR gRNA against SPAG9. (SPAG9-BGHcLV31-64)
- Product ID
- HB-8065
- Lot ID
- 59635738
- Culture start date
- 2021-09-17
- Culture harvest date
- 2021-09-29
Attribution
ENCODE4 project
- Lab
- Brenton Graveley, UConn
- Award PI
- Brenton Graveley, UConn
- Submitted by
- Xintao Wei
- Source
- ATCC
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:SPAG9-BGHcLV31-64
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM117FSW | deletion | repression | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAC
- Aliases
- encode:donor of HepG2, bradley-bernstein:Donor of HepG2
- Species
- Homo sapiens
- Life stage
- child
- Age
- 15 years
- Sex
- male
- Health status
- hepatocellular carcinoma
- Ethnicity
- European
- External resources
- References
Documents
general protocol
Description: Biosample protocol for sample SPAG9-BGHcLV31-64
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U41HG009889
immunoblot
Caption excerpt: Western blot following CRISPR against SPAG9 in HepG2 whole cell lysate using…
- Caption
- Western blot following CRISPR against SPAG9 in HepG2 whole cell lysate using SPAG9 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SPAG9. SPAG9 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U41HG009889