Experiment summary for ENCSR452TFV
doi:10.17989/ENCSR452TFV
Summary
- Status
- released
- Assay
- CRISPR genome editing followed by RNA-seq (CRISPR RNA-seq)
- Control type
- non-specific target
- Biosample summary
- Homo sapiens HepG2 genetically modified (deletion) using CRISPR
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Control CRISPR gRNA against no target in HepG2 cells followed by RNA-seq. (NT-BGHcLV31)
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 9.5 [1-1]9.6 [2-1]
- Size range
- >200
- Extraction method
- Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR156CIL
- Platform
- Illumina NovaSeq 6000
Attribution
ENCODE4 project
- Lab
- Brenton Graveley, UConn
- Award
- U41HG009889 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:NT-BGHcLV31
- Date released
- October 27, 2022
- Gene Silencing Series
- ENCSR714BYM , ENCSR254YPW , ENCSR764UVM , ENCSR143VRA , ENCSR312QIX , ENCSR636LSV , ENCSR424OIS , ENCSR441GUL , ENCSR048ILP , ENCSR648NMQ , ENCSR010WDI , ENCSR586DOD , ENCSR420PTK , ENCSR037RRC , ENCSR210JCP
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens HepG2 cell line genetically modified (deletion) using CRISPR | ENCBS426VMT | ENCGM474NKS | ENCLB518ERQ |
| 2 | 1 | Homo sapiens HepG2 cell line genetically modified (deletion) using CRISPR | ENCBS143SBN | ENCGM474NKS | ENCLB439RFX |