Summary for ENCSR427OCU
Summary
- Status
- released
- Description
- Functional characterization series for CRISPRi/dCas9-KRAB-MGA1-MGA2 sparse-tiling proliferation screens at ~7500 cCREs around ~450 essential genes in K562
- Donor diversity
- single
- Assay
- proliferation CRISPR screen
- Biosample summary
- Homo sapiens K562, 21 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPRi (sgRNA)
- Diseases
- Not reported
- Treatments
- Puromycin
- Elements references
- Tiling modality
- sparse peak
Attribution
ENCODE4 project
- Lab
- Will Greenleaf, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- will-greenleaf:K562_LargeScale_DHS_Growth_dCas9-KRAB-MGA1-MGA2
- External resources
- None submitted
Experiments in ENCSR427OCU
Experiments
1 experiment
Accession | Assay | Examined loci | Biosample summary | Lab | Status | Cart |
|---|---|---|---|---|---|---|
| ENCSR509FZL | proliferation CRISPR screen | Homo sapiens K562, 21 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPRi (sgRNA) | Will Greenleaf, Stanford | released |
Control experiments
1 control experiment
Accession | Control type | Biosample summary | Lab | Status | Cart |
|---|---|---|---|---|---|
| ENCSR729ITW | control | Will Greenleaf, Stanford | released |