Functional Characterization Experiment summary for ENCSR742NIM

doi:10.17989/ENCSR742NIM

Summary

Status
released
Assay
MPRA (Control MPRA)
Control type
control
Biosample summary
Homo sapiens HepG2 genetically modified using transduction
Biosample Type
cell line
Replication type
isogenic
Description
Elements cloned upstream of the reporter's minimal promoter, with barcode located in the 3' UTR of a reporter in a lentiviral vector, using a wild type integrase to allow genomic integration. One of nine methods testing element activity in parallel.
Nucleic acid type
DNA
Size range
10-20
Fragmentation methods
chemical (Illumina TruSeq)
Strand specificity
Strand-specific (unstranded)
Elements references
Elements mapping
ENCSR638VYO

Attribution

ENCODE4 project
Lab
Nadav Ahituv, UCSF
Award
UM1HG009408 (Jay Shendure, UW)
Project
ENCODE
Aliases
nadav-ahituv:53_WT-mpra-DNA
Date submitted
December 2, 2020
Date released
February 19, 2021

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11HepG2 cell line genetically modified (insertion) using transductionENCBS153VNEENCGM176VZRENCLB161MBK
31HepG2 cell line genetically modified (insertion) using transductionENCBS154QNZENCGM176VZRENCLB464SLO
21HepG2 cell line genetically modified (insertion) using transductionENCBS637OCXENCGM176VZRENCLB846OJU