Functional Characterization Experiment summary for ENCSR642ECM
doi:10.17989/ENCSR642ECM
Summary
- Status
- released
- Assay
- MPRA (Control MPRA)
- Control type
- control
- Biosample summary
- Homo sapiens HepG2 genetically modified (insertion) using transient transfection
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Elements cloned into the ORI (STARR-seq lacking a minimal promoter) vector, with both elements and barcodes cloned into the 3' UTR. Tested in an independent, second batch as ORI, and serves as an internal control to ORI_full.
- Nucleic acid type
- DNA
- Size range
- 10-20
- Fragmentation methods
- chemical (Illumina TruSeq)
- Platform
- Illumina NextSeq 500
- Elements references
- Elements mappings
Attribution
ENCODE4 project
- Lab
- Nadav Ahituv, UCSF
- Award
- UM1HG009408 (Jay Shendure, UW)
- Project
- ENCODE
- Aliases
- nadav-ahituv:ORI_b2-mpra-DNA
- Date submitted
- December 2, 2020
- Date released
- February 19, 2021
- Functional Characterization Series
- ENCSR033VBW
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 3 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transient transfection | ENCBS265FPZ | ENCGM517RIK | ENCLB318RQD |
| 1 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transient transfection | ENCBS646KCG | ENCGM517RIK | ENCLB191CCD |
| 2 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transient transfection | ENCBS824DAY | ENCGM517RIK | ENCLB668KAW |