Functional Characterization Experiment summary for ENCSR642ECM

doi:10.17989/ENCSR642ECM

Summary

Status
released
Assay
MPRA (Control MPRA)
Control type
control
Biosample summary
Homo sapiens HepG2 genetically modified using transient transfection
Biosample Type
cell line
Replication type
isogenic
Description
Elements cloned into the ORI (STARR-seq lacking a minimal promoter) vector, with both elements and barcodes cloned into the 3' UTR. Tested in an independent, second batch as ORI, and serves as an internal control to ORI_full.
Nucleic acid type
DNA
Size range
10-20
Fragmentation methods
chemical (Illumina TruSeq)
Strand specificity
Strand-specific (unstranded)
Elements references
Elements mapping
ENCSR638VYO

Attribution

ENCODE4 project
Lab
Nadav Ahituv, UCSF
Award
UM1HG009408 (Jay Shendure, UW)
Project
ENCODE
Aliases
nadav-ahituv:ORI_b2-mpra-DNA
Date submitted
December 2, 2020
Date released
February 19, 2021

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
31HepG2 cell line genetically modified (insertion) using transient transfectionENCBS265FPZENCGM517RIKENCLB318RQD
11HepG2 cell line genetically modified (insertion) using transient transfectionENCBS646KCGENCGM517RIKENCLB191CCD
21HepG2 cell line genetically modified (insertion) using transient transfectionENCBS824DAYENCGM517RIKENCLB668KAW