Functional Characterization Experiment summary for ENCSR193FCX

doi:10.17989/ENCSR193FCX

Summary

Status
released
Assay
CRISPR screen (Control CRISPR screen)
Control type
control
Biosample summary
Homo sapiens DU 145 genetically modified (insertion) using transduction, using CRISPRi (pgRNA) for multiple loci
Biosample Type
cell line
Perturbation type
CRISPRi
Tiling modality
full tiling
Replication type
isogenic
Description
Growth-based CRISPR screen targeting genomic regions near MYC and MYB genes.
Treatments
2 μg/mL Puromycin (CHEBI:17939) for 2 days, 0.2 μg/mL Puromycin (CHEBI:17939) for 2 days
Nucleic acid type
RNA
Size range
710-720
Strand specificity
unstranded
Controls
Elements references

Attribution

ENCODE4 project
Lab
Bing Ren, UCSD
Award
UM1HG009402 (Yin Shen, UCSF)
Project
ENCODE
Aliases
bing-ren:BC19E_D0_DU145
Date submitted
January 31, 2020
Date released
August 6, 2020
Functional Characterization Series
ENCSR112MYY 

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens DU 145 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (pgRNA) 0 days after the sample was treated with 2 μg/mL Puromycin for 2 days, 0.2 μg/mL Puromycin for 2 daysENCBS729PSFENCGM554RTS, ENCGM353IMQENCLB168IMK
21Homo sapiens DU 145 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (pgRNA) 0 days after the sample was treated with 2 μg/mL Puromycin for 2 days, 0.2 μg/mL Puromycin for 2 daysENCBS445JSIENCGM554RTS, ENCGM353IMQENCLB783GUI