ENCBS445JSI / cell line

Summary

Status
released
Term name
DU 145
Summary
Homo sapiens DU 145 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (pgRNA) 0 days after the sample was treated with 2 μg/mL Puromycin for 2 days, 0.2 μg/mL Puromycin for 2 days
Culture start date
2019-05-25
Culture harvest date
2019-07-10

Attribution

ENCODE4 project
Lab
Bing Ren, UCSD
Award PI
Yin Shen, UCSF
Submitted by
Bin Li
Source
ATCC
Project
ENCODE
External resources
Aliases
bing-ren:BC19B_DU145_D0_B2_BC120

Treatment details

Treatment
2 μg/mL Puromycin (CHEBI:17939) for 2 days
Type
chemical
Purpose
selection
Treatment
0.2 μg/mL Puromycin (CHEBI:17939) for 2 days
Type
chemical
Purpose
selection

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM554RTSinsertionexpressiontransduction
ENCGM353IMQCRISPRicharacterizationCRISPR

Donor information

Status
released
Accession
ENCDO842XEO
Aliases
encode:donor of DU 145, encode:donor of DU145, encode:donor of DU-145
Species
Homo sapiens
Life stage
adult
Age
69 years
Sex
male
Health status
Prostate carcinoma
Ethnicity
European
External resources