Experiment summary for ENCSR990RKL

Summary

Status
released
Assay
ChIP-seq
Target
Control
Biosample summary
Caenorhabditis elegans strain IG1654 (frSi12[pNP157(akir-1p::AKIR-1::GFP)]II) whole organism young adult (70 hours) at L1 larva starved after bleaching stage genetically modified using CRISPR
Biosample Type
whole organisms
Replication type
unreplicated
Description
This is the ChIP-seq control of transgenic worms expressing akir-1-eGFP fusion proteins.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:AKIR-1_IG1654_yAd-INPUT
Date submitted
June 11, 2018
Date released
June 13, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsLibrary
11strain IG1654 (frSi12[pNP157(akir-1p::AKIR-1::GFP)]II) mixed sex young adult (70 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using CRISPR targeting eGFP-akir-1ENCBS862HVBENCGM524HUGENCLB346MFP