ENCGM524HUG
Summary
- Status
- released
- Description
- A single copy transgene inserted in the ttTi5605 Mos1 position using CRISPR (IG1654 wt; frSi12[pNP157(akir-1p::AKIR-1::GFP)] II). The vector, pNP157 was created by Gibson Assembly using a portion of pAP087 along with the AKIR-1 region, ce11:chrII:4137162-4140534 with a c-terminal GFP tag.
- Type
- insertion
- Tags
- eGFP — C-terminal
- Purpose
- tagging
Modification site
- Target
- akir-1-celegans
- Coordinates
- ce11 chrII:8420207-8420207
Modification method
- Method
- CRISPR
- Guide RNAs
- ATATCAGTCTGTTTCGTAA