Experiment summary for ENCSR905NIK
doi:10.17989/ENCSR905NIK
Superseded by ENCSR939BTN
Supersedes ENCSR697GLD
Summary
- Status
- archived
- Assay
- CRISPR genome editing followed by RNA-seq (CRISPR RNA-seq)
- Target
- SRSF4
- Biosample summary
- Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens SRSF4
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- RNA-seq on K562 cells treated with a CRISPR gRNA against SRSF4. (SRSF4-BGKcLV04-B)
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 9.4 [2-1]9.6 [1-1]
- Size range
- >200
- Extraction method
- Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Platform
- Illumina HiSeq 2500
- Controls
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award
- U54HG007005 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:SRSF4-BGKcLV04-B
- Date submitted
- November 20, 2017
- Date released
- December 9, 2017
- Gene Silencing Series
- ENCSR506QBE
- Submitter comment
- As of May 24th, 2018, this experiment has superseded experiment ENCSR697GLD. As of November 2022, this dataset is superseded by ENCSR939BTN, which includes spike-ins.
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens K562 cell line genetically modified (deletion) using CRISPR targeting H. sapiens SRSF4 | ENCBS578FNZ | ENCGM183XQP | ENCLB889RIG |
| 1 | 1 | Homo sapiens K562 cell line genetically modified (deletion) using CRISPR targeting H. sapiens SRSF4 | ENCBS217XMV | ENCGM183XQP | ENCLB565YRN |