Experiment summary for ENCSR891IMI
doi:10.17989/ENCSR891IMI
Summary
- Status
- released
- Assay
- ChIA-PET
- Target
- POLR2A
- Biosample summary
- Homo sapiens activated T-cell female adult (41 years) treated with 50 U/mL Interleukin-2 for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours
- Biosample Type
- primary cell
- Replication type
- unreplicated
- Description
- RNAPII ChIA-PET in CD3_Activated_donor3
- Treatments
- 50 U/mL Interleukin-2 (UniProtKB:P60568) for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours
- Nucleic acid type
- DNA
- Size range
- 300-600
- Lysis method
- SDS
- Fragmentation methods
- sonication (Branson Sonifier 250)
- Size selection method
- BluePippin
- Platform
- Illumina NovaSeq 6000
Attribution
ENCODE4 project
- Lab
- Charles Lee
- Award
- 3UM1HG009409-04S1 (Charles Lee)
- Project
- ENCODE
- External resources
- Aliases
- charles-lee:CD3_Activated_donor3_RNAPII
- Date released
- June 29, 2022
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Antibody | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens female adult (41 years) activated T-cell primary cell treated with 50 U/mL Interleukin-2 for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours | ENCBS755WRA | ENCAB725WBH | ENCLB792MUR |