ENCAB725WBH

Antibody against Homo sapiens POLR2A

Homo sapiens
GM12878
characterized to standards with exemption
Status
released
Source (vendor)
BioLegend
Product ID
664912
Lot ID
B233183
Host
mouse
Clonality
monoclonal
Purification
affinity
Isotype
IgG2a
Antigen description
Clone 8WG16 recognizes YSPTSPS in the C-terminal domain which is conserved in wheat, yeast, mouse, c. elegans, x. laevis, and most eukaryotic RNAPII.
Aliases
yijun-ruan:pol2_antibody

Characterizations

POLR2A (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment from submitter
exempt from standards
Caption
Characterizations for this antibody were exempted for all cell types and tissues by the Antibody Review Panel on March 30, 2016.
Submitter comment
This is a well characterized antibody already used in many ENCODE experiments (clone 8WG16).
Reviewer comment
Characterizations for this antibody were exempted for all cell types and tissues by the Antibody Review Panel on March 30, 2016.
Submitted by
Idan Gabdank
Lab
Yijun Ruan, JAX
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POLR2A (Homo sapiens)
GM12878
Method: immunoblot
Attachment from submitter
exempt from standards
Caption
Primary characterization (e.g. immunoblot, immunoprecipitation) for the specified cell types and tissues are exempted for this antibody by the ENCODE antibody review panel because it is a well-characterized monoclonal antibody (clone 8WG16) that has been in use without issue by the community for many years.
Submitter comment
This is a well characterized antibody already used in many ENCODE experiments. It is also previously known as Convance cat# MMS-126R: http://www.biolegend.com/purified-anti-rna-polymerase-ii-rpb1-antibody-11666.html (clone 8WG16)
Reviewer comment
This is a well characterized antibody already used in many ENCODE experiments.
Submitted by
Idan Gabdank
Lab
Yijun Ruan, JAX
Download