ENCAB725WBH

Antibody against Homo sapiens POLR2A

Homo sapiens
K562, CD4-positive naive resting alpha-beta T cell, activated CD8-positive, alpha-beta T cell, CD8-positive naive resting alpha-beta T cell, GM12878, activated CD4 positive, naive alpha-beta T cell, activated CD4-positive, alpha-beta memory T cell, activated CD4-positive, alpha-beta T cell, activated CD8-positive, naive alpha-beta T cell, activated CD8-positive, alpha-beta memory T cell, CD8-positive, alpha-beta memory T cell, MCF-7, activated B cell, HepG2, RWPE1, B cell, CD8-positive, alpha-beta T cell, CD4-positive, alpha-beta memory T cell, AG04449, HEK293, BJ, AG04450, CD4-positive, alpha-beta T cell, common myeloid progenitor, CD34-positive, SK-N-SH, GM10248, MCF 10A, HEK293T, A549, endothelial cell of umbilical vein, HCT116
characterized to standards with exemption
Status
released
Source (vendor)
BioLegend
Product ID
664912
Lot ID
B233183
Characterized targets
POLR2A (Homo sapiens)
Host
mouse
Clonality
monoclonal
Purification
affinity
Isotype
IgG2a
Antigen description
Clone 8WG16 recognizes YSPTSPS in the C-terminal domain which is conserved in wheat, yeast, mouse, c. elegans, x. laevis, and most eukaryotic RNAPII.
Aliases
yijun-ruan:pol2_antibody
External resources

Characterizations

POLR2A (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment from submitter
exempt from standards
Caption
Characterizations for this antibody were exempted for all cell types and tissues by the Antibody Review Panel on March 30, 2016.
Submitter comment
This is a well characterized antibody already used in many ENCODE experiments (clone 8WG16).
Reviewer comment
Characterizations for this antibody were exempted for all cell types and tissues by the Antibody Review Panel on March 30, 2016.
Submitted by
Idan Gabdank
Lab
Yijun Ruan, JAX
Download
POLR2A (Homo sapiens)
K562CD4-positive naive resting alpha-beta T cellactivated CD8-positive, alpha-beta T cellCD8-positive naive resting alpha-beta T cellGM12878activated CD4 positive, naive alpha-beta T cellactivated CD4-positive, alpha-beta memory T cellactivated CD4-positive, alpha-beta T cellactivated CD8-positive, naive alpha-beta T cellactivated CD8-positive, alpha-beta memory T cellCD8-positive, alpha-beta memory T cellMCF-7activated B cellHepG2RWPE1B cellCD8-positive, alpha-beta T cellCD4-positive, alpha-beta memory T cellAG04449HEK293BJAG04450CD4-positive, alpha-beta T cellcommon myeloid progenitor, CD34-positiveSK-N-SHGM10248MCF 10AHEK293TGM12878A549endothelial cell of umbilical veinHCT116
Method: immunoblot
Attachment from submitter
exempt from standards
Caption
Primary characterization (e.g. immunoblot, immunoprecipitation) for the specified cell types and tissues are exempted for this antibody by the ENCODE antibody review panel because it is a well-characterized monoclonal antibody (clone 8WG16) that has been in use without issue by the community for many years.
Submitter comment
This is a well characterized antibody already used in many ENCODE experiments. It is also previously known as Convance cat# MMS-126R: http://www.biolegend.com/purified-anti-rna-polymerase-ii-rpb1-antibody-11666.html (clone 8WG16)
Reviewer comment
This is a well characterized antibody already used in many ENCODE experiments.
Submitted by
Idan Gabdank
Lab
Yijun Ruan, JAX
Download