Experiment summary for ENCSR741YCA
doi:10.17989/ENCSR741YCA
Summary
- Status
- released
- Assay
- shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
- Target
- TBRG4
- Biosample summary
- Homo sapiens HepG2 expressing RNAi targeting H. sapiens TBRG4
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- RNA-seq on HepG2 cells treated with an shRNA knockdown against TBRG4. (TBRG4_BGHLV27)
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 9.8 [1-1]9.6 [2-1]
- Size range
- >200
- Extraction method
- Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR449DXG
- Platform
- Illumina HiSeq 2500
- Controls
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award
- U54HG007005 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- References
- Aliases
- brenton-graveley:TBRG4_BGHLV27
- Date submitted
- January 22, 2016
- Date released
- February 11, 2016
- Gene Silencing Series
- ENCSR330RCO
- Submitter comment
- As of October 31st, 2017, the lab has corrected its genetic modification identifier (from "TRCN0000154962" to the correct ID, "TRCN0000155887").
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens TBRG4 | ENCBS049RJI | ENCGM626OBI | ENCLB522HIE |
| 2 | 1 | Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens TBRG4 | ENCBS501FGJ | ENCGM626OBI | ENCLB078QKF |