Experiment summary for ENCSR246RRQ
doi:10.17989/ENCSR246RRQ
Summary
- Status
- released
- Assay
- shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
- Control type
- non-specific target
- Biosample summary
- Homo sapiens HepG2 expressing RNAi
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Control shRNA against no target in HepG2 cells followed by RNA-seq. (non-target_BGHLV27)
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 9.8
- Size range
- >200
- Extraction method
- Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR449DXG
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award
- U54HG007005 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:non-target_BGHLV27
- Date submitted
- January 23, 2016
- Date released
- February 11, 2016
- Gene Silencing Series
- ENCSR692CHB , ENCSR016FXK , ENCSR330RCO , ENCSR969FIX , ENCSR610MSU , ENCSR789DBN , ENCSR597NVC , ENCSR224WRQ
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens HepG2 cell line expressing RNAi | ENCBS811GQK | ENCGM266AWP | ENCLB167EXT |
| 2 | 1 | Homo sapiens HepG2 cell line expressing RNAi | ENCBS129BNW | ENCGM266AWP | ENCLB946ZUF |