Experiment summary for ENCSR710NWE

doi:10.17989/ENCSR710NWE

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
IGF2BP3
Biosample summary
Homo sapiens HepG2 expressing RNAi targeting H. sapiens IGF2BP3
Biosample Type
cell line
Replication type
isogenic
Description
shRNA knockdown against IGF2BP3 in HepG2 cells followed by RNA-seq. (IGF2BP3-BGHLV12)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
8.9 [2-1]9.4 [1-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
Aliases
brenton-graveley:IGF2BP3-BGHLV12
Date submitted
September 29, 2014
Date released
October 16, 2014
Gene Silencing Series
ENCSR362BLN 
Tags
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Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens IGF2BP3ENCBS040DESENCGM605RKKENCLB069LNY
11Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens IGF2BP3ENCBS641AVIENCGM605RKKENCLB364DPW