Experiment summary for ENCSR237YZT
doi:10.17989/ENCSR237YZT
Summary
- Status
- released
- Assay
- shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
- Control type
- non-specific target
- Biosample summary
- Homo sapiens HepG2 expressing RNAi
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Control shRNA against no target in HepG2 cells followed by RNA-seq. (NT-BGHLV12-01-02)
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 8.9
- Size range
- >200
- Extraction method
- Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR449DXG
- Platform
- Illumina HiSeq 2000
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award
- U54HG007005 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:NT-BGHLV12-01-02
- Date submitted
- September 29, 2014
- Date released
- October 16, 2014
- Gene Silencing Series
- ENCSR983ZKO , ENCSR453OOG , ENCSR475UTA , ENCSR362BLN , ENCSR566IBA , ENCSR180ZGG , ENCSR608NVZ , ENCSR113GLI , ENCSR279BOZ , ENCSR663TDN , ENCSR105ARZ , ENCSR087ZNB , ENCSR875WKW , ENCSR605PVH , ENCSR355PLU , ENCSR106SDF , ENCSR398ILX , ENCSR263EJV , ENCSR220SFY
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
---|---|---|---|---|---|
1 | 1 | Homo sapiens HepG2 cell line expressing RNAi | ENCBS623BZF | ENCGM266AWP | ENCLB807KAY |
2 | 1 | Homo sapiens HepG2 cell line expressing RNAi | ENCBS638ASL | ENCGM266AWP | ENCLB781BJK |