Experiment summary for ENCSR550JJT
doi:10.17989/ENCSR550JJT
Summary
- Status
- released
- Assay
- ChIA-PET
- Target
- CTCF
- Biosample summary
- Homo sapiens activated naive CD4-positive, alpha-beta T cell male adult (43 years) treated with 10 ng/mL Interleukin-2 for 5 days, anti-CD3 and anti-CD28 coated beads for 7 days
- Biosample Type
- primary cell
- Replication type
- isogenic
- Description
- CTCF ChIA-PET in Naive_CD4_Activated_Day7
- Treatments
- 10 ng/mL Interleukin-2 (UniProtKB:P60568) for 5 days, anti-CD3 and anti-CD28 coated beads for 7 days
- Nucleic acid type
- DNA
- Size range
- 350-600
- Lysis method
- SDS
- Fragmentation methods
- sonication (Branson Sonifier 250)
- Size selection method
- BluePippin
- Platform
- Illumina MiSeq, Illumina HiSeq 4000
Attribution
ENCODE4 project
- Lab
- Yijun Ruan, JAX
- Award
- UM1HG009409 (Yijun Ruan, JAX)
- Project
- ENCODE
- External resources
- Aliases
- yijun-ruan:Naive_CD4_Activated_Day7_CTCF
- Date submitted
- August 3, 2020
- Date released
- July 21, 2021
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Antibody | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens male adult (43 years) activated naive CD4-positive, alpha-beta T primary cell treated with 10 ng/mL Interleukin-2 for 5 days, anti-CD3 and anti-CD28 coated beads for 7 days | ENCBS338LVO | ENCAB635SXP | ENCLB282YTU |
| 1 | 1 | Homo sapiens male adult (43 years) activated naive CD4-positive, alpha-beta T primary cell treated with 10 ng/mL Interleukin-2 for 5 days, anti-CD3 and anti-CD28 coated beads for 7 days | ENCBS716JOO | ENCAB635SXP | ENCLB825EVF |