ENCAB635SXP

Antibody against Homo sapiens CTCF

Homo sapiens
SK-N-SH, RWPE1
characterized to standards
Homo sapiens
GM12878, CD4-positive naive resting alpha-beta T cell, activated CD8-positive, alpha-beta T cell, activated CD4 positive, naive alpha-beta T cell, activated CD4-positive, alpha-beta memory T cell, activated CD4-positive, alpha-beta T cell, activated CD8-positive, alpha-beta memory T cell, CD8-positive, alpha-beta memory T cell, MCF-7, activated B cell, B cell, CD8-positive, alpha-beta T cell, CD4-positive, alpha-beta memory T cell, AG04449, HEK293, BJ, AG04450, CD4-positive, alpha-beta T cell, common myeloid progenitor, CD34-positive, GM10248, MCF 10A, HEK293T, A549, IMR-90, endothelial cell of umbilical vein, HCT116
characterized to standards with exemption
Status
released
Source (vendor)
ABclonal
Product ID
A1133
Lot ID
A1133
Characterized targets
CTCF (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Recombinant protein of human CTCF
External resources

Characterizations

CTCF (Homo sapiens)
GM12878CD4-positive naive resting alpha-beta T cellactivated CD8-positive, alpha-beta T cellactivated CD4 positive, naive alpha-beta T cellactivated CD4-positive, alpha-beta memory T cellactivated CD4-positive, alpha-beta T cellactivated CD8-positive, alpha-beta memory T cellCD8-positive, alpha-beta memory T cellMCF-7activated B cellB cellCD8-positive, alpha-beta T cellCD4-positive, alpha-beta memory T cellAG04449HEK293BJAG04450CD4-positive, alpha-beta T cellcommon myeloid progenitor, CD34-positiveGM10248MCF 10AHEK293TA549IMR-90endothelial cell of umbilical veinHCT116
Method: immunoblot
Attachment from submitter
exempt from standards
Caption
Primary characterization (e.g. immunoblot, immunoprecipitation) for the specified cell types are exempted for this antibody by the ENCODE antibody review panel, because the antibody has satisfactory primary and secondary characterizations in two other cell types.
Submitter comment
This antibody was not characterized in other cell types due to lack of assays in the cell types with an approved CTCF antibody.
Reviewer comment
Primary characterization (e.g. immunoblot, immunoprecipitation) for the specified cell types are exempted for this antibody by the ENCODE antibody review panel.
Submitted by
Bonita Lam
Lab
Yijun Ruan, JAX
CTCF (Homo sapiens)
SK-N-SHRWPE1
Method: immunoprecipitation
compliant
Submitter comment
Approved by Eric Mendenhall from AB WG on 1/25/21.
Submitted by
Bonita Lam
Lab
Yijun Ruan, JAX
CTCF (Homo sapiens)
Method: ChIP-seq comparison
compliant
Caption
Comparison of CTCF Abclonal antibody in ChIA-PET assays with compliant CTCF antibody from ChIP-seq antibody both performed on RWPE-1 cells
Submitted by
Khine Lin
Lab
Yijun Ruan, JAX
Download
CTCF (Homo sapiens)
Method: ChIP-seq comparison
compliant
Caption
Comparison of CTCF Abclonal antibody in ChIA-PET assays with compliant CTCF antibody from ChIP-seq antibody both performed on SK-N-SH cells
Submitted by
Khine Lin
Lab
Yijun Ruan, JAX
Download