Experiment summary for ENCSR523FJT
doi:10.17989/ENCSR523FJT
Summary
- Status
- released
- Assay
- DNase-seq
- Biosample summary
- Homo sapiens A549 treated with 100 nM dexamethasone (agonist) for 12 hours
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- DNase-seq on A549 cell line treated with 100 nM dexamethasone for 12 hours.
- Treatments
- 100 nM dexamethasone (CHEBI:41879) for 12 hours
- Nucleic acid type
- DNA
- Library starting quantity
- 75ng [3-1]40ng [1-1]
- Size selection method
- gel
- Platform
- Illumina HiSeq 2500, Illumina HiSeq 2000
- Controls
Attribution
Genomics of Gene Regulation Project
- Lab
- Tim Reddy, Duke
- Award
- U01HG007900 (Tim Reddy, Duke)
- Project
- GGR
- External resources
- Aliases
- tim-reddy:t12_dnase_seq
- Date submitted
- December 16, 2015
- Date released
- May 5, 2016
- Treatment Time Series
- ENCSR482QFS
- Annotation (gkmsvm-model)
- Submitter comment
- DNase-seq libraries were sequenced with four barcodes per lane to generate 50 bp single-end reads, of which only the first 20 bp were genomic DNA due to MmeI digestion (see Song and Crawford 2010).
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
---|---|---|---|---|
3 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 12 hours | ENCBS091SYA | ENCLB541JIA |
5 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 12 hours | ENCBS652QEI | ENCLB333GBB |
1 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 12 hours | ENCBS420ZHM | ENCLB153DUS |
2 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 12 hours | ENCBS097ZMG | ENCLB229HPB |