Experiment summary for ENCSR457RNA
doi:10.17989/ENCSR457RNA
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- hlh-13
- Biosample summary
- Caenorhabditis elegans strain OP744 (wgIs744 [hlh-13::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) whole organism late embryo (3.5 hours) using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans hlh-13
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic worms expressing hlh-13-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:HLH-13_OP744_LE
- Date submitted
- May 30, 2018
- Date released
- June 12, 2018
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Caenorhabditis elegans strain OP744 (wgIs744 [hlh-13::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex late embryo (3.5 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans hlh-13 | ENCBS747QFX | ENCGM593PZT | ENCAB728YTO | ENCLB702DCN |
| 2 | 1 | Caenorhabditis elegans strain OP744 (wgIs744 [hlh-13::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex late embryo (3.5 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans hlh-13 | ENCBS000CGI | ENCGM593PZT | ENCAB728YTO | ENCLB566SFB |