ENCBS747QFX / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP744 (wgIs744 [hlh-13::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex late embryo (3.5 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans hlh-13
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to HLH-13.
Life stage
late embryonic
Age
3.5 hours
Synchronization timepoint
egg bleaching

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:HLH-13_GFP_OP744_LE_Rep1

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM593PZTinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO670UNA
Aliases
robert-waterston:OP744
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
wgIs744 [hlh-13::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)
Sex
mixed
Strain name
OP744
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale