Experiment summary for ENCSR432JLI
doi:10.17989/ENCSR432JLI
Summary
- Status
- released
- Assay
- RIP-seq
- Target
- snRNP-U1-70K
- Biosample summary
- Drosophila melanogaster strain Oregon-R S2R+ stably expressing snRNP-U1-70K treated with 0.35 mM copper(II) sulfate for 24 hours
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- RIP-seq targeting snRNP-U1-70K in Drosophila S2R+ cells.
- Treatments
- 0.35 mM copper(II) sulfate (CHEBI:23414) for 24 hours
- Nucleic acid type
- RNA
- Size range
- >200
- Fragmentation methods
- chemical (generic)
- Size selection method
- AMPure XP bead purification
- Strand specificity
- unstranded
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
modENCODE Project
- Lab
- Brenton Graveley, UConn
- Award
- U01HG004271 (Susan Celniker, LBNL)
- Project
- modENCODE
- External resources
- References
- Aliases
- brenton-graveley:S2R+-CG8749-HA_RIP, modencode:5428
- Date submitted
- March 31, 2014
- Date released
- March 31, 2014
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Drosophila melanogaster strain Oregon-R S2R+ cell line stably expressing C-terminal FLAG-tagged snRNP-U1-70K under MtnA promoter, C-terminal HA-tagged snRNP-U1-70K under MtnA promoter treated with 0.35 mM copper(II) sulfate for 24 hours | ENCBS552ENG | ENCGM194EHB | ENCAB639FUH | ENCLB215ODF |
| 2 | 1 | Drosophila melanogaster strain Oregon-R S2R+ cell line stably expressing C-terminal FLAG-tagged snRNP-U1-70K under MtnA promoter, C-terminal HA-tagged snRNP-U1-70K under MtnA promoter treated with 0.35 mM copper(II) sulfate for 24 hours | ENCBS937EMZ | ENCGM194EHB | ENCAB639FUH | ENCLB269TZL |