Experiment summary for ENCSR385QCA

doi:10.17989/ENCSR385QCA

Summary

Status
released
Assay
RIP-seq
Target
HA
Control type
control
Biosample summary
Drosophila melanogaster strain Oregon-R S2R+ stably expressing HA treated with 0.35 mM copper(II) sulfate for 24 hours
Biosample Type
cell line
Replication type
isogenic
Description
RIP-seq empty vector control in S2R+ cells.
Treatments
0.35 mM copper(II) sulfate (CHEBI:23414) for 24 hours
Nucleic acid type
RNA
Size range
>200
Fragmentation methods
chemical (generic)
Size selection method
AMPure XP bead purification
Strand specificity
unstranded

Attribution

modENCODE Project
Lab
Brenton Graveley, UConn
Award
U01HG004271 (Susan Celniker, LBNL)
Project
modENCODE
References
Aliases
brenton-graveley:S2R+-Empty Vector_RIP, modencode:5430
Date submitted
March 31, 2014
Date released
March 31, 2014

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
41Drosophila melanogaster strain Oregon-R S2R+ cell line stably expressing C-terminal FLAG-tagged HA under MtnA promoter, C-terminal HA-tagged HA under MtnA promoter treated with 0.35 mM copper(II) sulfate for 24 hoursENCBS437QNCENCGM843AJGENCAB639FUHENCLB274GGQ
31Drosophila melanogaster strain Oregon-R S2R+ cell line stably expressing C-terminal FLAG-tagged HA under MtnA promoter, C-terminal HA-tagged HA under MtnA promoter treated with 0.35 mM copper(II) sulfate for 24 hoursENCBS727TWQENCGM843AJGENCAB639FUHENCLB917DVM
51Drosophila melanogaster strain Oregon-R S2R+ cell line stably expressing C-terminal FLAG-tagged HA under MtnA promoter, C-terminal HA-tagged HA under MtnA promoter treated with 0.35 mM copper(II) sulfate for 24 hoursENCBS866VOGENCGM843AJGENCAB639FUHENCLB602TNQ
11Drosophila melanogaster strain Oregon-R S2R+ cell line stably expressing C-terminal FLAG-tagged HA under MtnA promoter, C-terminal HA-tagged HA under MtnA promoter treated with 0.35 mM copper(II) sulfate for 24 hoursENCBS612RWZENCGM843AJGENCAB639FUHENCLB919UPY
71Drosophila melanogaster strain Oregon-R S2R+ cell line stably expressing C-terminal FLAG-tagged HA under MtnA promoter, C-terminal HA-tagged HA under MtnA promoter treated with 0.35 mM copper(II) sulfate for 24 hoursENCBS058XTVENCGM843AJGENCAB639FUHENCLB874XDH
21Drosophila melanogaster strain Oregon-R S2R+ cell line stably expressing C-terminal FLAG-tagged HA under MtnA promoter, C-terminal HA-tagged HA under MtnA promoter treated with 0.35 mM copper(II) sulfate for 24 hoursENCBS160PDGENCGM843AJGENCAB639FUHENCLB038NGB
61Drosophila melanogaster strain Oregon-R S2R+ cell line stably expressing C-terminal FLAG-tagged HA under MtnA promoter, C-terminal HA-tagged HA under MtnA promoter treated with 0.35 mM copper(II) sulfate for 24 hoursENCBS085GBTENCGM843AJGENCAB639FUHENCLB888GHI