Experiment summary for ENCSR395HQT

Summary

Status
released
Assay
CRISPR genome editing followed by RNA-seq (CRISPR RNA-seq)
Control type
non-specific target
Biosample summary
Homo sapiens K562 genetically modified using CRISPR
Biosample Type
cell line
Replication type
isogenic
Description
Control CRISPR gRNA against no target in K562 cells followed by RNA-seq on day 30. (NT-D30_BGKcLV02)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.9 [1-1]9.8 [2-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific
Spike-ins datasets
ENCSR358OMW

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
Aliases
brenton-graveley:NT-D30_BGKcLV02
Date submitted
March 15, 2016
Date released
March 23, 2016
Tags
Search for all Experiment with internal tag ENCORE

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11K562 cell line genetically modified (deletion) using CRISPRENCBS864MCXENCGM474NKSENCLB036CEZ
21K562 cell line genetically modified (deletion) using CRISPRENCBS016SETENCGM474NKSENCLB910IJD