Experiment summary for ENCSR371ICA
doi:10.17989/ENCSR371ICA
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- T20F7.1
- Biosample summary
- Caenorhabditis elegans strain OP754 (wgIs754 [T20F7.1::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) whole organism L4 larva (48 hours) at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans T20F7.1
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic worms expressing t20f7.1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:T20F7.1_OP754_L4
- Date submitted
- May 30, 2018
- Date released
- June 12, 2018
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Caenorhabditis elegans strain OP754 (wgIs754 [T20F7.1::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex L4 larva (48 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans T20F7.1 | ENCBS112UXR | ENCGM394ZUL | ENCAB728YTO | ENCLB706INT |
| 2 | 1 | Caenorhabditis elegans strain OP754 (wgIs754 [T20F7.1::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex L4 larva (48 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans T20F7.1 | ENCBS658LCZ | ENCGM394ZUL | ENCAB728YTO | ENCLB721SJQ |