ENCBS112UXR / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP754 (wgIs754 [T20F7.1::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex L4 larva (48 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans T20F7.1
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to T20F7.1.
Life stage
L4 larva
Age
48 hours
Synchronization timepoint
L1 larva starved after bleaching

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:T20F7.1_GFP_OP754_L4_Rep1

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM394ZULinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO633CKI
Aliases
robert-waterston:OP754
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
wgIs754 [T20F7.1::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)
Sex
mixed
Strain name
OP754
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale