Experiment summary for ENCSR335LHS

doi:10.17989/ENCSR335LHS

Summary

Status
released
Assay
ATAC-seq
Biosample summary
Homo sapiens stimulated activated effector memory CD8-positive, alpha-beta T cell male adult (33 years) treated with 100 ng/mL Interleukin-12 subunit alpha for 72 hours, 100 ng/mL Interleukin-12 subunit beta for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours
Biosample Type
primary cell
Replication type
isogenic
Treatments
100 ng/mL Interleukin-12 subunit alpha (UniProtKB:P29459) for 72 hours, 100 ng/mL Interleukin-12 subunit beta (UniProtKB:P29460) for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours
Nucleic acid type
DNA
Size range
150-1000
Fragmentation methods
chemical (Tn5 transposase)

Attribution

ENCODE4 project
Lab
Michael Snyder, Stanford
Award
UM1HG009442 (Michael Snyder, Stanford)
Project
ENCODE
External resources
Aliases
michael-snyder:pAS-585, michael-snyder:pAS-h.CD8 Memory.Effector 72 hour T cell activation(Altius Donor 8)
Date submitted
August 25, 2021
Date released
September 9, 2021
Annotation (ChromBPNet-model)
Tags
Search for all Experiment with internal tag RegulomeDB_2_2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11Homo sapiens male adult (33 years) stimulated activated effector memory CD8-positive, alpha-beta T primary cell treated with 100 ng/mL Interleukin-12 subunit alpha for 72 hours, 100 ng/mL Interleukin-12 subunit beta for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hoursENCBS371TCOENCLB774LNE
21Homo sapiens male adult (33 years) stimulated activated effector memory CD8-positive, alpha-beta T primary cell treated with 100 ng/mL Interleukin-12 subunit alpha for 72 hours, 100 ng/mL Interleukin-12 subunit beta for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hoursENCBS257CBKENCLB355CYJ