Experiment summary for ENCSR335LHS
doi:10.17989/ENCSR335LHS
Summary
- Status
- released
- Assay
- ATAC-seq
- Biosample summary
- Homo sapiens stimulated activated effector memory CD8-positive, alpha-beta T cell male adult (33 years) treated with 100 ng/mL Interleukin-12 subunit alpha for 72 hours, 100 ng/mL Interleukin-12 subunit beta for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours
- Biosample Type
- primary cell
- Replication type
- isogenic
- Treatments
- 100 ng/mL Interleukin-12 subunit alpha (UniProtKB:P29459) for 72 hours, 100 ng/mL Interleukin-12 subunit beta (UniProtKB:P29460) for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours
- Nucleic acid type
- DNA
- Size range
- 150-1000
- Fragmentation methods
- chemical (Tn5 transposase)
- Platform
- Illumina NovaSeq 6000
Attribution
ENCODE4 project
- Lab
- Michael Snyder, Stanford
- Award
- UM1HG009442 (Michael Snyder, Stanford)
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:pAS-585, michael-snyder:pAS-h.CD8 Memory.Effector 72 hour T cell activation(Altius Donor 8)
- Date submitted
- August 25, 2021
- Date released
- September 9, 2021
- Annotation (ChromBPNet-model)
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
---|---|---|---|---|
1 | 1 | Homo sapiens male adult (33 years) stimulated activated effector memory CD8-positive, alpha-beta T primary cell treated with 100 ng/mL Interleukin-12 subunit alpha for 72 hours, 100 ng/mL Interleukin-12 subunit beta for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours | ENCBS371TCO | ENCLB774LNE |
2 | 1 | Homo sapiens male adult (33 years) stimulated activated effector memory CD8-positive, alpha-beta T primary cell treated with 100 ng/mL Interleukin-12 subunit alpha for 72 hours, 100 ng/mL Interleukin-12 subunit beta for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours | ENCBS257CBK | ENCLB355CYJ |