ENCBS371TCO / primary cell
Summary
- Status
- released
- Term name
- stimulated activated effector memory CD8-positive, alpha-beta T cell
- Term ID
- NTR:0000668
- Summary
- Homo sapiens male adult (33 years) stimulated activated effector memory CD8-positive, alpha-beta T primary cell treated with 100 ng/mL Interleukin-12 subunit alpha for 72 hours, 100 ng/mL Interleukin-12 subunit beta for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours
- Separated from biosample
- ENCBS684DOK
Attribution
ENCODE4 project
- Lab
- Michael Snyder, Stanford
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Tao Wang
- Source
- StemExpress
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:pB-13531, michael-snyder:pB-h.CD8.Memory.Effector 72 hour T cell activation (CD3-CD28 beads) + 50U-mL IL-12 (Altius Donor 8) rep 2
Treatment details
- Treatment
- 100 ng/mL Interleukin-12 subunit alpha (UniProtKB:P29459) for 72 hours
- Type
- protein
- Purpose
- stimulation
- Treatment
- 100 ng/mL Interleukin-12 subunit beta (UniProtKB:P29460) for 72 hours
- Type
- protein
- Purpose
- stimulation
- Treatment
- anti-CD3 and anti-CD28 coated beads for 72 hours
- Type
- antibody
- Purpose
- activation
Donor information
- Status
- released
- Accession
- ENCDO830ROH
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 33 years
- Sex
- male
- External resources
- Submitter comment
- StemExpress:D001005067
Documents
cell isolation protocol
Description excerpt: Isolation of Immune cells by Stemcell Technologies Magnetic Beads kits and…
- Description
- Isolation of Immune cells by Stemcell Technologies Magnetic Beads kits and stimulations (Stamatoyannopoulos lab). This protocol is an updated version of : https://www.encodeproject.org/documents/b94d1a22-1ef2-4820-9b71-cdcfba41127c/
- Submitted by
- Jennifer Jou
- Lab
- John Stamatoyannopoulos, UW
- Grant
- UM1HG009444