Experiment summary for ENCSR182HOA

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Caenorhabditis elegans strain OP742 (wgIs742 [C02F12.10::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) whole organism L1 larva (6 hours) at L1 larva starved after bleaching stage genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic worms expressing c02f12.10-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:C02F12.10_OP742_L1
Date submitted
June 11, 2018
Date released
June 12, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
21strain OP742 (wgIs742 [C02F12.10::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex L1 larva (6 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-ceh-63ENCBS993TQPENCGM417BXOENCAB728YTOENCLB811QRA
11strain OP742 (wgIs742 [C02F12.10::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex L1 larva (6 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-ceh-63ENCBS294QADENCGM417BXOENCAB728YTOENCLB888JJI