Experiment summary for ENCSR182HOA
doi:10.17989/ENCSR182HOA
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- ceh-63
- Biosample summary
- Caenorhabditis elegans strain OP742 (wgIs742 [C02F12.10::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) whole organism L1 larva (6 hours) at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans ceh-63
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic worms expressing c02f12.10-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:C02F12.10_OP742_L1
- Date submitted
- June 11, 2018
- Date released
- June 12, 2018
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 2 | 1 | Caenorhabditis elegans strain OP742 (wgIs742 [C02F12.10::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex L1 larva (6 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans ceh-63 | ENCBS993TQP | ENCGM417BXO | ENCAB728YTO | ENCLB811QRA |
| 1 | 1 | Caenorhabditis elegans strain OP742 (wgIs742 [C02F12.10::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex L1 larva (6 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans ceh-63 | ENCBS294QAD | ENCGM417BXO | ENCAB728YTO | ENCLB888JJI |