ENCBS294QAD / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP742 (wgIs742 [C02F12.10::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex L1 larva (6 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-ceh-63
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to C02F12.10.
Life stage
L1 larva
Age
6 hours
Synchronization timepoint
L1 larva starved after bleaching

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
Aliases
valerie-reinke:C02F12.10_GFP_OP742_L1_Rep1

Genetic modifications

AccessionCategoryPurposeMethodSite
ENCGM417BXOinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO839KWC
Aliases
robert-waterston:OP742
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
wgIs742 [C02F12.10::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)
Sex
mixed
Strain name
OP742

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale