Experiment summary for ENCSR154MAJ

doi:10.17989/ENCSR154MAJ

Summary

Status
released
Assay
ChIA-PET
Target
CTCF
Biosample summary
Homo sapiens activated CD4-positive, alpha-beta memory T cell male adult (43 years) treated with anti-CD3 and anti-CD28 coated beads for 36 hours
Biosample Type
primary cell
Replication type
isogenic
Description
CTCF ChIA-PET in Memory_CD4_Activated_Day2
Treatments
anti-CD3 and anti-CD28 coated beads for 36 hours
Nucleic acid type
DNA
Size range
350-600
Lysis method
SDS
Fragmentation methods
sonication (Branson Sonifier 250)
Size selection method
BluePippin

Attribution

ENCODE4 project
Lab
Yijun Ruan, JAX
Award
UM1HG009409 (Yijun Ruan, JAX)
Project
ENCODE
External resources
Aliases
yijun-ruan:Memory_CD4_Activated_Day2_CTCF
Date submitted
August 3, 2020
Date released
July 21, 2021

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Antibody
Library
11Homo sapiens male adult (43 years) activated CD4-positive, alpha-beta memory T primary cell treated with anti-CD3 and anti-CD28 coated beads for 36 hoursENCBS844TVFENCAB635SXPENCLB530JEV
21Homo sapiens male adult (43 years) activated CD4-positive, alpha-beta memory T primary cell treated with anti-CD3 and anti-CD28 coated beads for 36 hoursENCBS397TSLENCAB635SXPENCLB590WVA