Experiment summary for ENCSR118XYK

doi:10.17989/ENCSR118XYK

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
PUM2
Biosample summary
Homo sapiens K562 expressing RNAi targeting H. sapiens PUM2
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on K562 cells treated with an shRNA knockdown against PUM2. (PUM2-BGKLV25)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.8
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:PUM2-BGKLV25
Date submitted
January 22, 2016
Date released
January 12, 2016
Gene Silencing Series
ENCSR064PGJ 
Tags
Visit collection page for ENCORESearch for all Experiment with internal tag ENCYCLOPEDIAv1Search for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21Homo sapiens K562 cell line expressing RNAi targeting H. sapiens PUM2ENCBS790JBMENCGM653YFDENCLB161QBJ
11Homo sapiens K562 cell line expressing RNAi targeting H. sapiens PUM2ENCBS071UNKENCGM653YFDENCLB403XUO