Experiment summary for ENCSR620PUP
doi:10.17989/ENCSR620PUP
Summary
- Status
- released
- Assay
- shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
- Control type
- non-specific target
- Biosample summary
- Homo sapiens K562 expressing RNAi
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Control shRNA against no target in K562 cells followed by RNA-seq. (NT_BGKLV25)
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 9.9 [2-1]10 [1-1]
- Size range
- >200
- Extraction method
- Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR449DXG
- Platform
- Illumina HiSeq 2000
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award
- U54HG007005 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:NT_BGKLV25
- Date submitted
- December 8, 2015
- Date released
- January 12, 2016
- Gene Silencing Series
- ENCSR051ACD , ENCSR485ZZS , ENCSR203XSC , ENCSR777OSI , ENCSR341IXU , ENCSR064PGJ , ENCSR628KJM , ENCSR294TLQ , ENCSR791AGL , ENCSR980RTI , ENCSR158YIO , ENCSR449VKZ , ENCSR849GEE
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens K562 cell line expressing RNAi | ENCBS217DFO | ENCGM266AWP | ENCLB454ECB |
| 1 | 1 | Homo sapiens K562 cell line expressing RNAi | ENCBS262OLW | ENCGM266AWP | ENCLB256QBT |