Experiment summary for ENCSR057TFD
doi:10.17989/ENCSR057TFD
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- gmeb-2
- Biosample summary
- Caenorhabditis elegans strain OP622 (unc-119(tm4063) III; wgIs622) whole organism late embryo 5 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans gmeb-2
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing GMEB-2-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000, Illumina HiSeq 2000
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:GMEB-2-OP622-LE
- Date submitted
- October 4, 2016
- Date released
- October 11, 2016
- Submitter comment
- ENCFF260ADO, ENCFF047JHQ, ENCFF512GZU, ENCFF393BYS were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 2 | 1 | Caenorhabditis elegans strain OP622 (unc-119(tm4063) III; wgIs622) mixed sex late embryo 5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans gmeb-2 | ENCBS131MCF | ENCGM398XZO | ENCAB728YTO | ENCLB905NZY |
| 1 | 1 | Caenorhabditis elegans strain OP622 (unc-119(tm4063) III; wgIs622) mixed sex late embryo 5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans gmeb-2 | ENCBS413KVJ | ENCGM398XZO | ENCAB728YTO | ENCLB063JQK |