Experiment summary for ENCSR057TFD

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Caenorhabditis elegans strain OP622 (unc-119(tm4063) III; wgIs622) whole organism late embryo 5 hours post synchronization using egg bleaching genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing GMEB-2-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation method
sonication (generic microtip)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:GMEB-2-OP622-LE
Date submitted
October 4, 2016
Date released
October 11, 2016
Submitter comment
ENCFF260ADO, ENCFF047JHQ, ENCFF512GZU, ENCFF393BYS were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
21strain OP622 (unc-119(tm4063) III; wgIs622) mixed sex late embryo 5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-gmeb-2ENCBS131MCFENCGM398XZOENCAB728YTOENCLB905NZY
11strain OP622 (unc-119(tm4063) III; wgIs622) mixed sex late embryo 5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-gmeb-2ENCBS413KVJENCGM398XZOENCAB728YTOENCLB063JQK