ENCBS131MCF / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP622 (unc-119(tm4063) III; wgIs622) mixed sex late embryo 5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting gmeb-2
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to GMEB-2
Life stage
late embryonic
Age
Unknown
Synchronization timepoint
egg bleaching + 5.0 hours

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
Aliases
valerie-reinke:GMEB-2_GFP_OP622_LE_Rep2

Genetic modifications

Accession
Category
Purpose
Method
Site
ENCGM398XZOinsertiontaggingbombardment
Coordinates
ce11 chrX:15855521-15889553
Integration site
random

Strain information

Status
released
Accession
ENCDO360AJB
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
unc-119(tm4063) III; wgIs622
Sex
mixed
Strain name
OP622

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale
general protocol
Description: Protocol used by the Reinke lab for ChIP-seq
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale