ENCBS131MCF / whole organisms
Summary
- Status
- released
- Term name
- whole organism
- Term ID
- UBERON:0000468
- Summary
- Caenorhabditis elegans strain OP622 (unc-119(tm4063) III; wgIs622) mixed sex late embryo 5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans gmeb-2
- Description
- Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to GMEB-2
- Life stage
- late embryonic
- Age
- Unknown
- Synchronization timepoint
- egg bleaching + 5.0 hours
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award PI
- Robert Waterston, UW
- Submitted by
- Alec Victorsen
- Source
- Valerie Reinke
- Project
- modERN
- External resources
- Aliases
- valerie-reinke:GMEB-2_GFP_OP622_LE_Rep2
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM398XZO | insertion | tagging | bombardment |
|
Strain information
- Status
- released
- Accession
- ENCDO360AJB
- Donor external identifiers
- Species
- Caenorhabditis elegans
- Genotype
- unc-119(tm4063) III; wgIs622
- Sex
- mixed
- Strain name
- OP622
- External resources
Documents
immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
- Caption
- DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
- Submitted by
- Alec Victorsen
- Lab
- Valerie Reinke, Yale
- Grant
- U41HG007355
general protocol
Description: Protocol used by the Reinke lab for ChIP-seq
- Submitted by
- Alec Victorsen
- Lab
- Valerie Reinke, Yale
- Grant
- U41HG007355