Experiment summary for ENCSR034VBA

doi:10.17989/ENCSR034VBA

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
HNRNPUL1
Biosample summary
Homo sapiens K562 expressing RNAi targeting H. sapiens HNRNPUL1
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on K562 cells treated with an shRNA knockdown against HNRNPUL1. (HNRNPUL1_BGKLV28)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.9 [2-1]9.8 [1-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:HNRNPUL1_BGKLV28
Date submitted
March 4, 2016
Date released
March 16, 2016
Gene Silencing Series
ENCSR478HBC 
Tags
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Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21Homo sapiens K562 cell line expressing RNAi targeting H. sapiens HNRNPUL1ENCBS997MUAENCGM474OMOENCLB258JXF
11Homo sapiens K562 cell line expressing RNAi targeting H. sapiens HNRNPUL1ENCBS511UGFENCGM474OMOENCLB926ODV