ENCBS867ESG / cell line
Summary
- Status
- released
- Term name
- SK-N-SH
- Term ID
- EFO:0003072
- Summary
- Homo sapiens SK-N-SH cell line genetically modified (insertion) using CRISPR targeting H. sapiens PPARA treated with 6 μM all-trans-retinoic acid for 48 hours
- Separated from biosample
- ENCBS982QWT
- Parent of biosamples
- ENCBS055BET, ENCBS062KGP
Attribution
ENCODE4 project
- Lab
- Michael Snyder, Stanford
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Nathaniel Watson
- Source
- Michael Snyder
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:pB-2589, michael-snyder:pB-eGFP-PPARA SK-N-SH
Treatment details
- Treatment
- 6 μM all-trans-retinoic acid (CHEBI:15367) for 48 hours
- Type
- chemical
- Purpose
- activation
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
---|---|---|---|---|---|
ENCGM195KLL | insertion | tagging | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000ABD
- Aliases
- encode:donor of SK-N-SH, bradley-bernstein:Donor of SK-N-SH cells, encode:donor of SH-SY5Y
- Species
- Homo sapiens
- Life stage
- child
- Age
- 4 years
- Sex
- female
- Health status
- Metastatic neuroblastoma from bone marrow
- External resources
- References
Documents
immunoblot
- Caption
- Immunoprecipitation was performed on nuclear extracts from biosample ENCBS982QWT (SK-N-SH wild type) cells using anti-eGFP antibody. The image shows western blot analysis of input (lane 1), and immunoprecipitate (lane 2). Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD).
- Lab
- Documents
transfection protocol
- Description
- SK-N-SH transfection protocol - Version 1 (Replicates split at T-75 stage, 200 µg/ml G418)
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996