ENCBS788WCK / cell line

Status: released
Term name: A549
Term ID: EFO:0001086
Summary: Homo sapiens A549 cell line genetically modified (insertion) using CRISPR targeting H. sapiens EHF
Culture harvest date: 2018-03-19
Separated from biosample: ENCBS756TFE

Accession	Category	Purpose	Method	Nucleic acid delivery method	Site
ENCGM432JBD	insertion	tagging	CRISPR		Target EHF-human

Status: released
Accession: ENCDO000AAZ
Aliases: encode:donor of A549, bradley-bernstein:Donor of A549 cells, john-stamatoyannopoulos:ATCC_A549
Species: Homo sapiens
Life stage: adult
Age: 58 years
Sex: male
Ethnicity: European
External resources: GEO:SAMN05733878
References: PMID:4357758

Caption excerpt: Immunoprecipitation was performed on nuclear extracts from biosample…

Caption: Immunoprecipitation was performed on nuclear extracts from biosample ENCBS788WCK (A549 eGFP-EHF replicate 1) cells using anti-eGFP antibody. The image shows western blot analysis of input (lane 1), immunoprecipitate (lane 2), and mock immunoprecipitate using IgG (lane 3). Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD). The target molecular weight is 63.0 kD as indicated with an arrow. Lower size bands which may be due to potential degradation products are marked with asterisks.
Submitted by: Nathaniel Watson
Lab: Michael Snyder, Stanford
Grant: UM1HG009442
Documents: michael-snyder:E4_P3_IP-WB_v2

Description: Cell growth protocol for A549 cells (version 1).

Description excerpt: A549 transfection protocol - Version 1 (Replicates split right after…

Description: A549 transfection protocol - Version 1 (Replicates split right after transfection, 500 µg/ml G418)
Submitted by: Nathaniel Watson
Lab: Michael Snyder, Stanford
Grant: U54HG006996