ENCBS769VOW / cell line

Summary

Status: released
Term name: SK-N-SH
Term ID: EFO:0003072
Summary: Homo sapiens SK-N-SH cell line genetically modified (insertion) using CRISPR targeting H. sapiens CSDC2 treated with 6 μM all-trans-retinoic acid for 48 hours

Attribution

Lab: Michael Snyder, Stanford
Award PI: Michael Snyder, Stanford
Submitted by: Nathaniel Watson
Source: Michael Snyder
Project: ENCODE
External resources: GEO:SAMN29769906
Cellosaurus:CVCL_0531
Aliases: michael-snyder:pB-2608, michael-snyder:pB-eGFP-CSDC2 SK-N-SH input

Treatment details

Treatment: 6 μM all-trans-retinoic acid (CHEBI:15367) for 48 hours
Type: chemical
Purpose: activation

Pooled from biosamples

2 biosamples

Accession	Type	Term	Summary
ENCBS377IYO	cell line	SK-N-SH	Homo sapiens SK-N-SH cell line genetically modified (insertion) using CRISPR targeting H. sapiens CSDC2 treated with 6 μM all-trans-retinoic acid for 48 hours
ENCBS948IUU	cell line	SK-N-SH	Homo sapiens SK-N-SH cell line genetically modified (insertion) using CRISPR targeting H. sapiens CSDC2 treated with 6 μM all-trans-retinoic acid for 48 hours

Genetic modifications

Accession	Category	Purpose	Method	Nucleic acid delivery method	Site
ENCGM678TAK	insertion	tagging	CRISPR		Target CSDC2-human

Donor information

Status: released
Accession: ENCDO000ABD
Aliases: encode:donor of SK-N-SH, bradley-bernstein:Donor of SK-N-SH cells, encode:donor of SH-SY5Y
Species: Homo sapiens
Life stage: child
Age: 4 years
Sex: female
Health status: Metastatic neuroblastoma from bone marrow
External resources: GEO:SAMN05733880
References: PMID:4748425

Documents

Description: Cell growth protocol for SK-N-SH cells (version 1).

Attachment PDF Icon

ENCODE4_Snyder_CellGrowth_SK-N-SH_v1.pdf

Submitted by: Nathaniel Watson
Lab: Michael Snyder, Stanford
Grant: UM1HG009442

Description excerpt: SK-N-SH transfection protocol - Version 2 (Replicates split right after…

Attachment PDF Icon

ENCODE4_Snyder_CellTransfection_SK-N-SH_v2.pdf

Description: SK-N-SH transfection protocol - Version 2 (Replicates split right after transfection, 200 µg/ml G418)
Submitted by: Nathaniel Watson
Lab: Michael Snyder, Stanford
Grant: UM1HG009442

Description: RA treatment protocol for SK-N-SH cells (version 1).

Attachment PDF Icon

ENCODE4_Snyder_RATreatment_SK-N-SH_v1.pdf

Submitted by: Nathaniel Watson
Lab: Michael Snyder, Stanford
Grant: U54HG006996