ENCBS582KTC / cell line

Summary

Status
released
Term name
C4-2B
Summary
Homo sapiens C4-2B cell line expressing RNAi targeting Non-specific target control
Description
C4-2B cells treated with a siRNA knockdown against a non-specific target control, used in RNA-seq experiments.

Attribution

ENCODE3 project
Lab
Peggy Farnham, USC
Award PI
Michael Snyder, Stanford
Submitted by
Heather Witt
Project
ENCODE
External resources
Aliases
peggy-farnham:B-C4-2B-siRNA-control-rep3

Genetic modifications

AccessionCategoryPurposeMethodSite
ENCGM752AFWinterferencerepressionRNAi

Donor information

Status
released
Accession
ENCDO000ACX
Aliases
encode:donor of C4-2B, encode:donor of LNCaP clone FGC, encode:donor of LNCAP
Species
Homo sapiens
Life stage
adult
Age
50 years
Sex
male
Health status
prostate adenocarcinoma
Ethnicity
Caucasian
External resources
References

Documents

transfection protocol
Description: Basic transfection protoco with Dharmafectl from Dharmacon
Submitted by
Heather Witt
Lab
Peggy Farnham, USC
other
Description: Basic siRNA suspension protocol from Dharmacon
Submitted by
Heather Witt
Lab
Peggy Farnham, USC