ENCBS567LZT / cell line
Summary
- Status
- released
- Term name
- GM23338
- Term ID
- EFO:0007950
- Summary
- Homo sapiens GM23338 cell line genetically modified (insertion) using CRISPR targeting H. sapiens CREB1
- Separated from biosample
- ENCBS683AJJ
- Parent of biosamples
- ENCBS883HUT, ENCBS299EEL
Attribution
ENCODE4 project
- Lab
- Michael Snyder, Stanford
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Nathaniel Watson
- Source
- Michael Snyder
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:pB-2617, michael-snyder:pB-eGFP-CREB1 PGP1
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
---|---|---|---|---|---|
ENCGM827WTQ | insertion | tagging | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO336AAA
- Aliases
- bradley-bernstein:Donor hu43860C, encode:donor of hu43860C
- Donor external identifiers
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 53 years
- Sex
- male
- Ethnicity
- European
- External resources
Documents
immunoblot
- Caption
- Immunoprecipitation was performed on nuclear extracts from biosample ENCBS683AJJ (GM23338 wild type) cells using anti-eGFP antibody. The image shows western blot analysis of input (lane 1), and immunoprecipitate (lane 2). Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD).
- Lab
- Documents
transfection protocol
- Description
- PGP1 transfection protocol - Version 1 (Replicates split right after transfection, 30 µg/ml G418, mTeSR medium)
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996