ENCBS883HUT / cell line

Status: released
Term name: GM23338
Term ID: EFO:0007950
Summary: Homo sapiens GM23338 cell line genetically modified (insertion) using CRISPR targeting H. sapiens CREB1
Culture harvest date: 2018-03-08
Separated from biosample: ENCBS567LZT

Lab: Michael Snyder, Stanford
Award PI: Michael Snyder, Stanford
Submitted by: Nathaniel Watson
Source: Michael Snyder
Project: ENCODE
External resources: GEO:SAMN18514161
Cellosaurus:CVCL_F182
Aliases: michael-snyder:pB-2619, michael-snyder:pB-eGFP-CREB1 PGP1 bulk1 (rep2)

Accession	Category	Purpose	Method	Nucleic acid delivery method	Site
ENCGM827WTQ	insertion	tagging	CRISPR		Target CREB1-human

Caption excerpt: Immunoprecipitation was performed on nuclear extracts from biosample…

Caption: Immunoprecipitation was performed on nuclear extracts from biosample ENCBS883HUT (GM23338 eGFP-CREB1 replicate 1) cells using anti-eGFP antibody. The image shows western blot analysis of input (lane 1), immunoprecipitate (lane 2), and mock immunoprecipitate using IgG (lane 3). Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD). The target molecular weight is kD as indicated with an arrow.
Submitted by: Nathaniel Watson
Lab: Michael Snyder, Stanford
Grant: UM1HG009442
Documents: michael-snyder:E4_P3_IP-WB_v2

Description: Cell growth protocol for PGP1 cells (version 1).

Description excerpt: PGP1 transfection protocol - Version 1 (Replicates split right after…

Description: PGP1 transfection protocol - Version 1 (Replicates split right after transfection, 30 µg/ml G418, mTeSR medium)
Submitted by: Nathaniel Watson
Lab: Michael Snyder, Stanford
Grant: U54HG006996