ENCBS392AAA / cell line
Summary
- Status
- released
- Term name
- K562
- Term ID
- EFO:0002067
- Summary
- Homo sapiens K562 cell line stably expressing C-terminal eGFP-tagged NR4A1
- Description
- K562 cell line stably expressing a C-terminal LAP-tag containing eGFP, fused to NR4A1
- Culture harvest date
- 2012-12-13
- Passage number
- 5
Attribution
ENCODE3 project
- Lab
- Kevin White, UChicago
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Jay Rehm
- Source
- Kevin White
- Project
- ENCODE
- External resources
- Aliases
- kevin-white:NR4A1_GFP_1
- Submitter comment
- Biorep1
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
---|---|---|---|---|---|
ENCGM696LPO | insertion | tagging | stable transfection |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAD
- Aliases
- encode:donor of K562, bradley-bernstein:Donor of K562 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 53 years
- Sex
- female
- Health status
- chronic myelogenous leukemia (CML)
- External resources
- References
Documents
immunoblot
- Caption
- Western blot analysis of nuclear lysates prepared from a K562 stable cell line expressin eGFP-NR4A1 using the Roche anti-GFP 11814450001 antibody. The expected MW is 97.99, 99.53kDa.
- Submitted by
- Mark Murphy
- Lab
- Kevin White, UChicago
- Grant
- U54HG006996
immunoblot
- Caption
- Western blot analysis of nuclear lysates prepared from a K562 stable cell line expressing eGFP-NR4A1 using the Roche anti-GFP 11814450001 antibody. The expected MW is 97.99, 99.53kDa.
- Submitted by
- Jay Rehm
- Lab
- Kevin White, UChicago
- Grant
- U54HG006996
FACs analysis
- Caption
- eGFP-NR4A1 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby Stain from Life technologies, and sorted by FACS. Shown are the cells from the GFP+ fraction quantified for their GFP and nuclear signal colocalization.
- Submitted by
- Mark Murphy
- Lab
- Kevin White, UChicago
- Grant
- U54HG006996
FACs analysis
- Caption
- eGFP-NR4A1 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby Stain from Life technologies, and sorted by FACS. Shown are the cells from the GFP++ fraction quantified for their GFP and nuclear signal colocalization.
- Submitted by
- Mark Murphy
- Lab
- Kevin White, UChicago
- Grant
- U54HG006996
FACs analysis
- Caption
- eGFP-NR4A1 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby Stain from Life technologies, and sorted by FACS. Shown are the cells from the GFP+ fraction quantified for their GFP and nuclear signal colocalization.
- Submitted by
- Mark Murphy
- Lab
- Kevin White, UChicago
- Grant
- U54HG006996