ENCBS378VQG / whole organisms
Summary
- Status
- released
- Term name
- whole organism
- Term ID
- UBERON:0000468
- Summary
- Drosophila melanogaster strain Cad-GFP-7T embryo (0-4 hours) whole organism stably expressing C-terminal eGFP-tagged cad
- Life stage
- embryonic
- Age
- 0-4 hours
Attribution
modENCODE Project
- Lab
- Kevin White, UChicago
- Award PI
- Kevin White, UChicago
- Submitted by
- Kevin White
- Source
- Kevin White
- Project
- modENCODE
- Aliases
- kevin-white:B2-modencode_2626
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
---|---|---|---|---|---|
ENCGM690RNJ | insertion | tagging | stable transfection |
|
Strain information
- Status
- released
- Accession
- ENCDO070BAB
- Aliases
- modencode:Cad-GFP-7T, bloomington:30875
- Donor external identifiers
- Species
- Drosophila melanogaster
- Genotype
- y[1] w[*]; PBac{y[+mDint2] w[+mC]=cad-EGFP.S}VK00033
- Sex
- unknown
- Strain background
- VK00033
- Strain name
- Cad-GFP-7T
Documents
immunofluorescence
- Caption
- Strain name: Cad-GFP-7T Genotype: w[*]; PBac{y[+]-attP-3B}VK00033 Gene Target: cad Transgene: caudal genomic coding region with stop codon replaced with GFP Tag Type: LAP-TAG_EGFP Short Description: P[acman] BAC number CH322_115J21 has been chosen to target the gene caudal. The LAP-TAG containing the GFP has been recombineered in 3' of the gene in frame. The engineered vector has then been inserted into the specific docking site with the help of ATT sites. Recombinant individuals were screened based on mini-white presence. The line has been stabilized as homozygous for the transgene. Versatile P[acman] BAC libraries for transgenesis studies in Drosophila melanogaster. Koen J T Venken, Joseph W Carlson, Karen L Schulze, Hongling Pan, Yuchun He, Rebecca Spokony, Kenneth H Wan, Maxim Koriabine, Pieter J de Jong, Kevin P White, Hugo J Bellen & Roger A Hoskins. Nature Methods 6, 431 - 434 (2009) Vector Used: P[acman] Genomic Coordinates: 2L from 20765625 to 20786898 attP Coordinates: 3L:6,435,776..6,435,776 Made_by: Rebecca Spokony, Koen Venken
- Submitted by
- Alec Victorsen
- Lab
- Kevin White, UChicago
- Grant
- U01HG004264