ENCDO070BAB

Accession	Category	Purpose	Method	Nucleic acid delivery method	Site
ENCGM690RNJ	insertion	tagging		stable transfection	Target cad-dmelanogaster

Caption excerpt: Strain name: Cad-GFP-7T Genotype: w[*]; PBac{y[+]-attP-3B}VK00033 Gene…

Caption: Strain name: Cad-GFP-7T Genotype: w[*]; PBac{y[+]-attP-3B}VK00033 Gene Target: cad Transgene: caudal genomic coding region with stop codon replaced with GFP Tag Type: LAP-TAG_EGFP Short Description: P[acman] BAC number CH322_115J21 has been chosen to target the gene caudal. The LAP-TAG containing the GFP has been recombineered in 3' of the gene in frame. The engineered vector has then been inserted into the specific docking site with the help of ATT sites. Recombinant individuals were screened based on mini-white presence. The line has been stabilized as homozygous for the transgene. Versatile P[acman] BAC libraries for transgenesis studies in Drosophila melanogaster. Koen J T Venken, Joseph W Carlson, Karen L Schulze, Hongling Pan, Yuchun He, Rebecca Spokony, Kenneth H Wan, Maxim Koriabine, Pieter J de Jong, Kevin P White, Hugo J Bellen & Roger A Hoskins. Nature Methods 6, 431 - 434 (2009) Vector Used: P[acman] Genomic Coordinates: 2L from 20765625 to 20786898 attP Coordinates: 3L:6,435,776..6,435,776 Made_by: Rebecca Spokony, Koen Venken
Submitted by: Alec Victorsen
Lab: Kevin White, UChicago
Grant: U01HG004264