ENCBS275XXE / whole organisms

Status: released
Term name: whole organism
Term ID: UBERON:0000468
Summary: Drosophila melanogaster strain Abd-B-GFP embryo (4-20 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Abd-B
Description: Transgenic D. mel line, stably expressing a C-terminal GFP fused to Abd-B
Life stage: embryonic
Age: 4-20 hours

Accession	Category	Purpose	Method	Nucleic acid delivery method	Site
ENCGM357VTZ	insertion	tagging	site-specific recombination		Target Abd-B-dmelanogaster Coordinates dm3 chr3R:12749382-12848402

Caption excerpt: Strain name: Abd-B-GFP Genotype: w; PBac{y[+]-attP-3B}VK00037 Gene…

Caption: Strain name: Abd-B-GFP Genotype: w; PBac{y[+]-attP-3B}VK00037 Gene Target: Abd-B Transgene: Abd-B genomic coding region with stop codon replaced with GFP Tag Type: LAP-TAG_EGFP Short Description: P[acman] BAC number CH321-91P18 has been chosen to target the gene Abdominal-B. The LAP-TAG containing the GFP has been recombineered in 3' of the gene in frame. The engineered vector has then been inserted into the specific docking site with the help of ATT sites. Recombinant individuals were screened based on mini-white presence. The line has been stabilized as homozygous for the transgene. Vector Used: P[acman] Genomic Coordinates: 3R from 12749382 to 12848402 attP Coordinates: 2L:1,582,820..1,582,820 Made_by: Alec Victorsen, Rebecca Spokony
Submitted by: Alec Victorsen
Lab: Kevin White, UChicago
Grant: U01HG004264