ENCDO269BCO
Alternate accession: ENCDO523IWJ
Strain information
- Status
- released
- Accession
- ENCDO269BCO
- Aliases
- modencode:Abd-B-GFP, bloomington:38625
- Donor external identifiers
- Species
- Drosophila melanogaster
- Genotype
- w[1118]; PBac{y[+mDint2] w[+mC]=Abd-B-EGFP.S}VK00037/SM57
- Strain background
- VK00037
- Strain name
- Abd-B-GFP
- External resources
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM357VTZ | insertion | tagging | site-specific recombination |
|
Documents
immunofluorescence
Caption excerpt: Strain name: Abd-B-GFP
Genotype: w; PBac{y[+]-attP-3B}VK00037
Gene…
- Caption
- Strain name: Abd-B-GFP Genotype: w; PBac{y[+]-attP-3B}VK00037 Gene Target: Abd-B Transgene: Abd-B genomic coding region with stop codon replaced with GFP Tag Type: LAP-TAG_EGFP Short Description: P[acman] BAC number CH321-91P18 has been chosen to target the gene Abdominal-B. The LAP-TAG containing the GFP has been recombineered in 3' of the gene in frame. The engineered vector has then been inserted into the specific docking site with the help of ATT sites. Recombinant individuals were screened based on mini-white presence. The line has been stabilized as homozygous for the transgene. Vector Used: P[acman] Genomic Coordinates: 3R from 12749382 to 12848402 attP Coordinates: 2L:1,582,820..1,582,820 Made_by: Alec Victorsen, Rebecca Spokony
- Submitted by
- Alec Victorsen
- Lab
- Kevin White, UChicago
- Grant
- U01HG004264