ENCBS157LEK / whole organisms

Status: released
Term name: whole organism
Term ID: UBERON:0000468
Summary: Drosophila melanogaster strain Stat92E-GFP mixed sex embryo (0-12 hours) whole organism stably expressing C-terminal eGFP-tagged Stat92E
Description: Transgenic D. mel line, stably expressing a C-terminal GFP fused to Stat92E
Life stage: embryonic
Age: 0-12 hours

Accession	Category	Purpose	Method	Nucleic acid delivery method	Site
ENCGM383IXW	insertion	tagging		stable transfection	Target Stat92E-dmelanogaster

Caption excerpt: Strain name: Stat92E-GFP Genotype: PBac{y[+]-attP-3B}VK00037 Gene…

Caption: Strain name: Stat92E-GFP Genotype: PBac{y[+]-attP-3B}VK00037 Gene Target: Stat92E Transgene: Stat92E genomic coding region, stop codon replaced with GFP Tag Type: LAP-TAG_EGFP Short Description: P[acman] BAC number CH321-73F24 has been chosen to target the gene Signal-transducer and activator of transcription protein at 92E. The LAP-TAG containing the GFP has been recombineered in 3' of the gene in frame. The engineered vector has then been inserted into the specific docking site with the help of Att sites. Recombinant individuals were screened based on mini-white presence. The line has been stabilized as homozygous for the transgene. Vector Used: P[acman] Genomic Coordinates: 3R from 16326088 to 16413006 attP Coordinates: 2L:1582820..1582820 Made_by: Nader Jameel, Alec Victorsen, Rebecca Spokony
Submitted by: Alec Victorsen
Lab: Kevin White, UChicago
Grant: U01HG004264